anti-cheratina K7 monoclonale murino, Ks7.18, 1 ml

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

Ks7.18 represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas hepatocellular and prostate carcinomas are negative.

Detects specific subtypes of adenocarcinomas: adenocarcimona of pancreas, gallbladder, lung, cervix; cholangio carcinoma of liver; ductal and lobular carcinoma of breast; carcinomas of ovary ; transitional cell carcinoma of bladder; mesothelioma; negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed.

Reacts with Mr 54,000 polypeptide (keratin K7; formerly also designated cytokeratin 7) of human glandular epithelia. Reactivity on cultured cell lines: HeLa, RT 112, T24, BT-20, CAMA-1, Detroit 562, (MCF-7 and HT-29 are negative).

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: Ks7.18
Immunogen: cytoskeletal proteins from cultured HeLa cells
UniprotID: Q29S21 (bovine), P08729 (human), A0A287ASI0 (Pig), H0VIA2 (guinea pig), A0A6P7DW90
(sheep)
Synonym: keratin, type II cytoskeletal 7, cytokeratin-7, CK-7, keratin-7, K7, sarcolectin, type-II keratin Kb7,
KRT7, SCL
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, human, pig, sheep
No reactivity: dog, mouse, rabbit, rat

Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:50-1:250 (0.2-1 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:50-1:250 (0.2-1 μg/ml, protease treatment and/or microwave treatment recommended)
Western Blot (WB): 1:1,000-1:2,000 (25-50 ng/ml)