Glucose, peptone and yeast extract contained in the thioglycolate medium provide growth factors necessary for bacterial growth. The reducing agents sodium thioglycolate and L-cystine reduce the accumulation of peroxides, which are lethal to some microorganisms. Resazurin serves as a redox indicator; on oxidation it shows a pink colour, on reduction it is colourless.
Getest volgens artsenijboek | Ph. Eur. USP |
Casein peptone (pancreas hydrolysate) | 15 g/l |
Sodium chloride (NaCl) | 2.5 g/l |
Yeast extract | 5 g/l |
L-Cysteine | 0.5 g/l |
Glucose monohydrate | 5.5 g/l |
Sodium thioglycolate | 0.5 g/l |
Sodium resazurin | 0.001 g/l |
Agar-Agar | 0.75 g/l |
pH value (25 °C) | 6.9-7.3 |