For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system.
Molar extinction coefficient: 4,4x104 L x mol-1 x cm-1 (acc. to 0,667 L x mol-1 x cm-1 for a 0,1 % solution).
Albumin, very low endotoxin, NZ-Origin is particularly recommended for use during culturing of eukaryotic cells and in cell culture assays. This albumin of superior quality is thoroughly tested for its very low endotoxin content, therefore providing trouble-free cell culture assays and reliable results even when handling primary cells or stem cells.
Bovine serum albumin is basically suitable for stabilisation of all enzymes and antibodies, as well as for blocking of hybridisations and immunoassays.
Tested for very low endotoxin content.
BSA | Art. No. | Recommended Application |
Albumin Solution 20 %, Europe | 9400 | Stabilization of proteins in general, especially antibodies. Suitable for all biochemical assays, e. g. as protein standard or blocking reagent, unless special requirements apply. |
Albumin Solution 30 %, Europe | 9401 | Stabilization of proteins in general, especially antibodies. Suitable for all biochemical assays, e. g. as protein standard or blocking reagent, unless special requirements apply. |
Albumin Solution 30 %, with azide, US-Origin | 9414 | Stabilization of proteins in general, especially antibodies. Suitable for all biochemical assays, e. g. as protein standard or blocking reagent, unless special requirements apply. Recommended if albumin with certified origin USA has to be used. |
Fraction V, Europe | 1ETA | Stabilisation of proteins and blocking reagent in protein biochemical detection systems. Basically suitable for all assays unless specific requirements apply. |
Fraction V, US-Origin | 3854 | Stabilisation of proteins and blocking reagent in all standard biochemical detection systems. Basically suitable for all assays unless specific requirements apply. Recommended if albumin with certified origin USA has to be used. |
Fraction V, NZ-Origin | 8076 | Stabilisation of proteins and blocking reagent in protein biochemical detection systems. Basically suitable for all assays unless specific requirements apply. Recommended if albumin with certified origin New Zealand or Australia has to be used. |
Fraction V, pH 5.2, Europe | 2834 | Stabilisation of proteins and blocking reagent. Particularly recommended for Western-Blotting and ELISAs. |
Fraction V, pH 5.2, US-Origin | 1ET8 | Stabilisation of proteins and blocking reagent. Particularly recommended for Western-Blotting and ELISAs. Recommended if albumin with certified origin USA has to be used. |
Fraction V, sufhydryl-blocked, NZ-Origin | 1ET3 | Particularly sensitive assays where monomeric albumin is guaranteed to be used or where reproducibility is particularly important. Can be stored as monomers over a longer period of time. |
Fraction V, biotin-free, NZ-Origin | 0163 | Stabilisation of proteins and blocking reagent in all biotin / streptavidin-mediated detection systems (membrane-bound- or coating-plate-assays, as well as soluble assays). |
Fraction V, protease-free, Europe | T844 | Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays. |
Fraction V, protease-free, US-Origin | 1ET5 | Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays. Recommended if albumin with certified origin USA has to be used. |
Fraction V, protease-free, NZ-Origin | 1ET7 | Stabilisation of proteins in general, antibodies in particular, and blocking reagent in sensitive enzymatic detection systems, activity tests or protein/protein-interaction assays. Recommended if albumin with certified origin New Zealand or Australia has to be used. |
Fraction V, IgG-free, US-Origin | 1ET9 | Stabilisation of antibodies. Blocking reagent in all antibody-mediated detection systems and Radio-Immuno Assays (RIAs). Reduces false-positive signals. Recommended if albumin with certified origin USA has to be used. |
Fraction V, IgG-free, NZ-Origin | 3737 | Stabilisation of antibodies. Blocking reagent in all antibody-mediated detection systems and Radio-Immuno Assays (RIAs). Reduces false-positive signals. Recommended if albumin with certified origin New Zealand or Australia has to be used. |
Fraction V, fatty acid-free, Europe | 0052 | Stabilisation of proteins and blocking reagent in detection systems. Particularly recommended for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins. |
Fraction V, fatty acid-free, US-Origin | 9638 | Particularly for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins. Also well suited for cell biology and all antibody-mediated detection systems. Recommended if albumin with certified origin USA has to be used. |
Fraction V, fatty acid-free, NZ-Origin | 1ETC | Stabilisation of proteins and blocking reagent in detection systems. Particularly for experiments on metabolism proteins, membrane-associated proteins and cell surface proteins. Recommended if albumin with certified origin New Zealand or Australia has to be used. |
Fraction V, very low endotoxin, US-Origin | 1ET6 | Cell culture of all cell lines, primary cells, stem cells. Cloning and 3D-cultures or cell-stressing assays like transfections, in vitro infections, cell fusion. Recommended if albumin with certified origin USA has to be used. |
Fraction V, very low endotoxin, NZ-Origin | 1ET4 | Cell culture of all cell lines, primary cells, stem cells. Cloning and 3D-cultures or cell-stressing assays like transfections, in vitro infections, cell fusion. Recommended if albumin with certified origin New Zealand or Australia has to be used.. |
Molar extinction coefficient: 4,4x104 L x mol-1 x cm-1 (acc. to 0,667 L x mol-1 x cm-1 for a 0,1 % solution).
BSA are proteins yielded from blood, which function as binding and transport proteins in blood circulation. Depending on the preparation process, the albumins contain different quality and quantities of metabolic products, enzymes, peptides, fatty acids, vitamines, etc. and are suitable for different applications.
Heatshock albumin
For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system.
Albumin, NZ-Origin is particularly recommended when procedural regulations require the use of albumin of guaranteed origin Australia or New Zealand.
BSA, US-Origin is particularly recommended if the use of albumin with certified US-Origin is dictated by process regulations.
Albumin of European origin is produced from blood of European cattle herds. It is excellent for use in all assays and products manufactured for regions outside the USA, Australia and New Zealand.
Appearance | powder to flakes |
Colour | pale yellow to greenish |
Origin | Australia/New Zealand |
Assay | ≥98 % |
Protein | ≥98 % |
Loss on drying | ≤5 % |
Solution (4 % in H2O) | complies |
pH value (10 % in H2O) | 6.5-7.5 |
Sulphated ash | ≤2 % |
Heavy metals | ≤10 ppm |
Protease | none detected |
Endotoxin content | ≤3 E.U./mg |
Bioburden (TAMC) | ≤1000 cfu/g |