MRS Broth with low pH value. For cultivation of special strains of lactobacilli, which show reduced growth under higher pH-values. Also for efficient protein expression of many proteins of Lactobacillus. 0.1 g/l Magnesium sulphate (anhydrous) is equivalent to 0.2 g/l Magnesium sulphate heptahydrate. 0.003 g/l Manganese sulphate (anhydrous) is equivalent to 0.05 g/l Manganese sulphate tetrahydrate.
Casein peptone | 10 g/l |
Yeast extract | 4 g/l |
Beef extract | 10 g/l |
Glucose | 20 g/l |
Dipotassium phosphate | 2 g/l |
Sodium acetate | 5 g/l |
Triammonium citrate | 2 g/l |
Magnesium sulphate heptahydrate | 0,20 g/l |
Mangansulphate tetrahydrate | 0,05 g/l |
Polysorbate 80 | 1,08 g/l |
pH value | 5,7 ±0,1 |