Magnetic beads can be used as solid support phase in DNA extraction and purification protocols by a simple bind/wash/elute principle. The products in this category are intended for own development of protocols and are suitable for various sample sources and buffer systems. MagSi-beads for genomic applications are available with a range of physical properties and a silica or carboxyl modified surface.
Magnetic silica beads of 300 nm size with optimized magnetic content and long suspension time. Ultra-fast ferromagnetic silica particles with instant magnetic separation. Intended for nucleic acid isolation from various sources (blood, cells, bacteria etc.) for manual and automated work-flow.
Specific Application of Silica- and of Carboxylated Beads
Bead surface | Silica beads | Carboxylated beads |
Compatible Buffer Systems | Chaotropic buffers | PEG-based, low pH or chaotropic buffers |
Binding Mechanism | Precipitation with chaotropic salts | Precipitation by polymers like PEG, divalent cations (e.g. Mg2+) or chaotropic salts |
Elution | Low salt conditions | Low salt conditions or pH shift from acidic to alkalineconditions |
Recommended use | Recommended in case chaotropic conditions can be applied. | Recommended if flexibility in buffer systems is necessary or if non-chaotropic conditions have to be applied. Results in higher purity and recovery rate under non-chaotropic conditions. |
Concentration | 300 ±15 mg/ml |
d50 | 300 nm |
DNA yield (20 µl beads) | >3 µg |