Yeast Nitrogen Base (without amino acids) contains ammonium sulphate, all vitamins and all organic salts needed to cultivate yeasts, except for the amino acids and carbohydrate sources.
By selective addition of single amino acids and sugars the yeast cells are tested for sugar assimilation and for their dependency on specific amino acids, hereby revealing parameters for their classification.
Synonym | YNB medium |
Ammonium sulphate | 5,0 g/l |
Potassium phosphate | 1,0 g/l |
Magnesium sulphate | 0,5 g/l |
Calcium chloride | 0,1 g/l |
Sodium chloride (NaCl) | 0,1 g/l |
Inositol | 2,0 mg/l |
Boric acid | 0,5 mg/l |
Niacin | 0,4 mg/l |
Thiamine hydrochloride | 0,4 mg/l |
Pyridoxin hydrochloride | 0,4 mg/l |
Calcium pantothenate | 0,4 mg/l |
Manganese sulphate | 0,4 mg/l |
Zinc sulphate | 0,4 mg/l |
p-Aminobenzoic acid | 0,2 mg/l |
Iron chloride | 0,2 mg/l |
Riboflavine | 0,2 mg/l |
Sodium molybdate | 0,2 mg/l |
Potassium iodide | 0,1 mg/l |
Copper sulphate | 0,04 mg/l |
Biotin | 0,002 mg/l |
Folic acid | 0,002 mg/l |
pH value | 5,4 ±0,2 |