Perfect separation behaviour in the gel. Protein ladders from Carl ROTH are acylated and pre-reduced, for optimal running behaviour in the gel. For each batch, intensity of bands is optimised and electrophoretically tested. All protein ladders are offered as ready-to-use solutions which may be applied directly to the gel.
Please note: Reliable size determination of proteins cannot be performed using prestained markers. For this, we recommend the use of an unstained marker.
ROTI®Mark ALL BLUE is a ladder with 12 blue prestained proteins covering a range from 10 to 240 kDa in Tris-Glycine buffer. For better orientation in the gel the 25 kDa and the 75 kDa fragment are enhanced in their mass.
ROTI®Mark ALL BLUE is an ideal size ladder for controlling the gel run during SDS-PAGE and for reliable evaluation of transfer efficiency during Western Blot.
Fig.: 15 % SDS-PAGE (Tris-Glycine)
Aantal proteïnebanden | 12 |
Aantal sporen | ~200/ml |
Kleuring | blue prestained |
Groottebereik | 10-240 kDa |
Bewijs in de westernblot | no detection |
Kwantificering | non recommendé |
Verzending | ready-to-use |
Versterkte bande bij | 25 kD, 72 kD |
Marker | ALL BLUE | BI-PINK | TRICOLOR | TRICOLOR XTRA |
Art. No. | 2242 (fig. 1) | 8269 (fig. 2) | 8271 (fig. 3) | 2244 (fig. 4) |
Size Range (kD) | 10-240 | 10-175 | 10-245 | 10-310 |
Number of Fragments | 12 | 11 | 12 | 12 |
Reinforced band at (kD) | 25, 72 | - | - | - |
Quantitation | not recomm. | not recomm. | not recomm. | not recomm. |
Staining | blue prestained | pink prestained | blue prestained | blue prestained |
Additional reference bands | - | 10 kD, 40 kD, 90 kD blue | 25 kD green, 75 kD red | 25 kD green, 75 and 310 kD red |
Detection in Western-Blot | no detection | no detection | no detection | no detection |
Number of Lanes in minigel | 200 / ml | 200 / ml | 200 / ml | 200 / ml |
Delivery | ready-to-use | ready-to-use | ready-to-use | ready-to-use |