Agar medium used for selection and selective cultivation of recombinant, ampicillin resistant E. coli. With high salt content acc. to Luria/Miller.
The anhydrous powder contains ampicillin for an end concentration of 100 µg/ml.
Voordeel | High salt content acc. to Luria/Miller. With ampicillin: end concentration of 100 μg/ml. |
Tryptone | 10 g/l |
Yeast extract | 5 g/l |
Sodium chloride (NaCl) | 10 g/l |
Ampicillin | 0,1 g/l |
Agar-Agar | 15 g/l |
pH value | 7,0 ±0,2 |