This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: desmosomes of epithelial and myocardial cells (incl. cultured cells); zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells; adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin.
Polypeptide reacting: Mr 83,000 Plakoglobin, 'band 5' polypeptide of intercellular adhering junctions (identical to gamma-catenin).
Reactivity on cultured cell lines: cell cultures forming monolayers.
Host: mouse
Antibody type: monoclonal
Isotype: IgG2b
Clone: PG 5.1
Immunogen: plakoglobin, 'band 5' protein from bovine snout epidermis
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, dog, human, mouse, rat, zebrafish
Immunocytochemistry (ICC): 1:5 (10 μg/ml)
Immunohistochemistry (IHC) - frozen: 1:25-1:100 (0.5-2 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:25-1:100 (0.5-2 μg/ml, microwave treatment recommended)
Western Blot (WB): 1:500-1:2,000 (0.025-0.1 μg/ml)