anti-GST-tag mouse monoclonal, F50-3D12.2, 25 µg
SKU 1NN8.1
€ 125,40
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anti-GST-tag mouse monoclonal, F50-3D12.2, 25 µg
Product Details
P-phrases: P280 P302+P352 P304+P340 P305+P351+P338
H-phrases: H315-H319-H335
CAS number: 14933-09-06
Chemical formulas: C19H41NO3S
HS code: 30021500
Brand: PROGEN
anti-GST-tag mouse, monoclonal, F50-3D12.2, after dissolv. in 100 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Storage temp. +4 °C
Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
- Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
- High affinity to the respective protein tag
- Robust & highly specific detection of the fusion protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-GST-tag mouse monoclonal, F50-3D12.2 lyophilized, monoclonal, affinity purified
The monoclonal F50-3D12.2 antibody recognizes the GST-tag (Glutathione S-transferase). The GST-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.
Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: F50-3D12.2Immunogen: not available or unknown
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: WB
Reactivity: GST
Toepassing
Western Blot (WB): 1:500 (0.5 μg/ml)
Western blot analysis of E. coli lysate containing GST-tagged protein with anti-GST-tag antibody. Western blot analysis was performed on 100 ng or 25 ng of E. coli lysate containing GST-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-GST-tag mouse monoclonal, F50-3D12.2 (Cat. No. 910GSTL) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Geen medisch hulpmiddel / Geen ivD-product
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