Distilled water mixed with DEPC and steam sterilised. By DEPC Histidin residues in proteins are modified to N-carbethoxy histidin, resulting in inhibition of RNases and DNases. DEPC decomposes to CO2 and ethanol during sterilisation.
Also available as 50 reaction tubes or as 50 glass ampoules with 1 ml each nuclease free water in a tube rack.
To prevent loss of volume in the 1 mltubes (T143.4), we recommend freezing the product at -20°C after receipt.
After autoclaving, each lot is photometrically tested for optimal purity in the wave length range relevant for nucleic acid research.