Transfer membrane ROTI®NC 0.45, 20 × 20 cm, 5 Piece(s)

Transfer membrane for protein analyses, Southern- and Northern-Blots.

High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.

The pore size of 0,45 μm is suitable for proteins and peptides of all sizes. The best retention rate is achieved when blotting proteins of ≥20 kDa. Having a high protein binding capacity, the membrane not only efficiently prevents protein loss during transfer, but also guarantees very sensitive detection of even low protein amounts.

A slight optical difference between the two membrane sides results from the membrane production and has no influence on the binding capacity or the blot quality of the two sides. Both sides can be used equally for transfer and detection.We recommend to always use the optically same side as “detection side” to achieve maximum reproducibility.

Optimised for proteins ≥20 kDa. Western- and far-Western-blots, Southern- and Northern blots, colony- and plaque-lifting of nucleic acids and proteins, dot- and slot blots.
Detection via radio activity, chemoluminescence, colour.