Inhibitor cocktail for inhibition of standard proteases. Inhibits serine proteases, aminopeptidase B, leucine-aminopeptidases, metalloproteases, cysteine proteases, and aspartic proteases.
Recommended as cocktail for protease inhibition in extracts from bacteria.
Well soluble in DMSO and hygroscopic. The premixed powder may easily be reconstituted in 1 ml DMSO and may then be diluted with 4 ml distilled water to form a slightly opaque inhibitor stock-solution.
Recommended dilution approx. 1:40.
Concentrations in stock-solution are:
AEBSF: 20 mM
Bestatin: 1.7 mM
E-64: 200 μM
EDTA: 85 mM
Pepstatin A: 2 mM
DMSO (1 ml) for reconstitution of the lyophilized inhibitor mix is included.
Contents of this Kit may not be bought separately.
Aanbevolen verdunning | ~ 1:40 |
Gebruikstip | 1 vial is sufficient for inhibition of proteases from approx. 20 g E. coli. |
Appearance | white powder |
AEBSF hydrochloride | complies |
Bestatin | complies |
E-64 | complies |
EDTA-Na2 | complies |
Pepstatin A | complies |