Ready-to-use, gas-stabilized ready-to-use acrylamide solutions with different concentrations and ratios of acrylamide to bisacrylamide. The ROTIPHORESE® gel solutions are very easy to use and enable reproducible and high-resolution gel electrophoresis.
ROTIPHORESE® gel solutions can be used for protein and nucleic acid separation in PAGE gels. The high-purity solutions can be combined with any buffer system. During sequencing, they facilitate the preparation of sequencing gels of consistent quality; our fluorescence-free gel solutions are recommended for the use of automatic sequencers.
The ROTIPHORESE® acrylamide and bisacrylamide solutions from Carl ROTH are produced only from acrylic acid-free substances and are strictly controlled in acrylamide content and pH. Oxidation products are removed from the solution by a selective absorption process. Their new formation and the polymerization of the solution are prevented by stabilizing the solution with inert gas.
In order to ensure a high lot-to-lot consistency of our ROTIPHORESE® solutions, each batch is subjected to an electrophoretic test. Fluorescence-free gel solutions are tested for suitability for automatic sequencers.
Cross linking 2,7.
Safe
Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.
Superior quality
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.
The right choice for each and every application
The large variety of different ROTIPHORESE® solutions provides you with the right product for each and every application. ROTIPHORESE® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.
ROTIPHORESE®Gel 30 and Gel 40 are ready-to-use, gas-stabilised acrylamide solutions with different concentrations and with different ratios of acrylamide to bisacrylamide. ROTIPHORESE® ready-to-use solutions can be used directly.
Recommendation for Use of ROTIPHORESE® Ready-Made Gel Solutions
Analysis of | Gel solution | Art. No. |
Proteins/peptides >15 kDa, DNA fragments | Gel 30 (37,5:1) | 3029.1 |
Gel 40 (37,5:1) | T802.1 | |
Proteins/peptides <30 kDa and DNA oligonucleotides | Gel 40 (29:1) | A515.1 |
DNA sequences (manual sequencing) | ROTIPHORESE®DNA sequencing system | A431.1 |
Gel 40 (19:1) | 3030.1 | |
DNA sequences (short-run automat. sequencing) | NF solution 40 % (19:1) | A516.1 |
DNA sequences (long-run automat. sequencing) | NF solution 30 % (29:1) | A124.1 |
NF solution 40 % (29:1) | A121.1 |
Filled under protective gas., Each batch is functionally tested for its suitability in electrophoresis., Note: Product may crystallise. It can be liquefied by heating in a water bath to max. 40 °C. |