Buffer solution composed of PBS with the addition of the non-ionic detergent Tween® 20. Since detergents are known to reduce non-specific protein-protein interactions, PBST is preferably used as basic solution during Western blotting, ELISA or other immunochemical assays.
Prepared solution may be sterilized by filtration (0,22 µm). For long-term storage, buffer solution should be kept at +4 °C.
Menghoeveelheid | 10.05 g/l |
pHwaarde | 7,4 ±0,2 |
Prepared 1x PBST solution contains: 0.14 M NaCl, 2,7 mM KCl, 10 mM phosphate, 0.05 % Tween® 20, pH-value 7.4 ±0.2, when prepared in deionized or distilled water. |