ROTI®Black P is a kit for speedy, reproducible silver staining of proteins in free and foil-supported gels.
ROTI®Black P allows the detection of proteins from 1 ng/mm2 due to its high sensitivity in combination with the very low background of the stained gels. Free gels can be stained without methanol, for carrier-supported gels methanol is required.
The ROTI®Black P Kit comes with an easy to follow protocol that guarantees you reliable results.
Sodium thiosulphate (Art. No. L534), silver nitrate (Art. No. L535), empty test tubes (Art. No. L536), formaldehyde (Art. No. L537), and sodium carbonate (Art. No. L538).
Contents of this Kit may not be bought separately.
Shipped without racket.
Sensitiviteit | 1 |
Toepassing | Silver staining kit. Highly sensitive. Simple, fast and reproducible. |
Product | Sensitivity | Rapid Gel Analysis |
Gel Drying |
Elution/ Transfer |
2D- Gels |
MS- Analysis |
ROTI®Black P | 1 ng/mm2 | - | +++ | - | +++ | - |
ROTI®Blue | 10 ng/mm2 | - | +++ | +++ | - | +++ |
ROTI®Blue quick | 10 ng/mm2 | +++ | +++ | ++ | - | ++ |
ROTI®Blau R | 50 ng/mm2 | +++ | + | - | - | - |
+++: recommended +: suitable -: not recommended |
Summary Staining of Protein Gels
Product | Sensitivity | Description |
ROTI®Black P | 1 ng/mm2 | Silver staining kit. Highly sensitive. Simple, fast and reproducible. |
ROTI®Blue | 10 ng/mm2 | Coomassie®-solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible. |
ROTI®Blue quick | 10 ng/mm2 | Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics. |
ROTIPHORESE®Blau R | 50 ng/mm2 | Coomassie® staining solution concentrate for rapid staining. |
Coomassie® Blue G250 (C.I. 42655) | 60 ng/mm2 | Dye for performing Coomassie® staining. ODmax = 595 |
Coomassie® Blue R250 (C.I. 42660) | 50 ng/mm2 | Dye for performing Coomassie staining. ODmax = 550 |