Ponceau S (C.I. 27195), 10 g

Staining of NC membranes: 0.2-2 % Ponceau S in 3 % trichloroacetic acid (Art. No. 8789.1) + 3 % sulphosalicylic acid (Art. No. 4119.1), incubate for 1-5 mins. at room temperature. Enhance contrast by incubation in water acidified by a few drops acetic acid.

Staining of PVDF membranes: 0.1-1 % Ponceau S in 10 % acetic acid (Art. No. 6755.1), incubate 1-5 mins. at room temperature. Suitable for rapid control of transfer in Western blot, PVDF membrane should not be completely dried before immunodetection.

Prior to Western blot analysis decolourise in PBS (Art. No. 1058.1) or TBS (Art. No. 1060) for 10-20 mins. at room temperature.