Additional Washing Buffer which may be used with the kits MagSi-gDNA blood-, -saliva- or -Vegetal kit. It is not included in the three kits, but may be bought separately.
Wash Buffer III eliminates the need for a drying step to remove traces of ethanol, resulting in faster protocols and DNA with higher purity.
Specific Application of Silica- and of Carboxylated Beads
Bead surface | Silica beads | Carboxylated beads |
Compatible Buffer Systems | Chaotropic buffers | PEG-based, low pH or chaotropic buffers |
Binding Mechanism | Precipitation with chaotropic salts | Precipitation by polymers like PEG, divalent cations (e.g. Mg2+) or chaotropic salts |
Elution | Low salt conditions | Low salt conditions or pH shift from acidic to alkalineconditions |
Recommended use | Recommended in case chaotropic conditions can be applied. | Recommended if flexibility in buffer systems is necessary or if non-chaotropic conditions have to be applied. Results in higher purity and recovery rate under non-chaotropic conditions. |
pH value | 6,0-6,5 |