Magnetic silica particles with high quality streptavidin covalently bonded to the surface of the particle. Applications include immunoassays and capture or purification of biotinylated molecules.
Various types of this product are available, with different mean size, streptavidin coating chemistry, and binding capacity.
Bigger packages available on request.
Magnetic silica particles of 600 nm size with high quality streptavidin covalently attached to the bead surface.
Specific Application of Silica- and of Carboxylated Beads
Bead surface | Silica beads | Carboxylated beads |
Compatible Buffer Systems | Chaotropic buffers | PEG-based, low pH or chaotropic buffers |
Binding Mechanism | Precipitation with chaotropic salts | Precipitation by polymers like PEG, divalent cations (e.g. Mg2+) or chaotropic salts |
Elution | Low salt conditions | Low salt conditions or pH shift from acidic to alkalineconditions |
Recommended use | Recommended in case chaotropic conditions can be applied. | Recommended if flexibility in buffer systems is necessary or if non-chaotropic conditions have to be applied. Results in higher purity and recovery rate under non-chaotropic conditions. |
Concentration | 10±0,5 mg/ml |
d50 | 530-600 nm |
Bead number | 8-20 x 109 beads/ml |
Assay (Iron oxide) | 35-45 % |
Binding capacity (biotinylated fluorescein) | 3,5-5 nmol/mg |