Leupeptin competitively inhibits calpain, cathepsin B, kallikrein, papain, plasmin and trypsin. Inhibition of thrombin is controversely discussed. Little or non-inhibiting for pepsin, cathepsin A and D and chymotrypsin.
Working concentration: 0.5-50 µg/ml (1-100 µM). Working solutions are stabile only for few hours.
Due to its aldehyde groups, leupeptin may interfere with protein detection assays (e.g. Bradford).
Assay (HPLC) | ≥96.5 % |
Solution (1 % in H2O) | clear |