Transfer membrane for most nucleic acid and protein immobilising techniques
Replaces Protran® BA 85 from Whatman®
Multiple use - ideal for most nucleic acid and protein immobilising techniques. Pure nitro-cellulose, free from cellulose acetate and triton. They have a high binding capacity and low background.
Nitrocellulose should only conditionally be used for electrophoretic transfer of nucleic acids, because of the high salt concentrations required for the transfer.
Membranes of 0,45 μm pore size allow for DNA transfer (minimum 300 bp) and protein transfer (minimum 20 kDa).
Southern, Northern, Western transfer, colony and plaque hybridisation, immunoblots, nucleic acid and protein dot-blot, slot-blot, electrophoretic transfer of proteins.
Detection systems are radioactivity, chemoluminescence, colour
In practical roll format for maximum flexibility in cutting.
Dimensions | 400 × 30 cm |
Version | roll |
Pore size | 0,45 µm |
Type | Protran® NC 0,45 |
Bond | electrostatic, hydrophobic |
Binding capacity | 86-110 µg/cm² |
Immobilisation | UV, baking (max. 80 °C) |
Technical Parameters of ROTH-Transfer Membranes
Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
Roti® PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 140 ±20 | ≥125 | 0,2 | not necessary |
Roti® PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | ca. 200 | 0,45 | not necessary |
Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
Roti® NC | Nitrocellulose | electrostatic, hydrophobic | 150 ±50 | 125-200 | 0,2 | UV, baking (max. 80 °C) |
Roti® NC 0.45 | Nitrocellulose | electrostatic, hydrophobic | 150 ±10 | ≥125 | 0,45 | UV, baking (max. 80 °C) |
Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
Biomolecules / Membrane Pores | Size (kD) |
genomic DNA (human) | >11000000 |
E. coli | approx. 1500000 |
0.45 μm | approx. 5000 |
0.2 μm | approx. 2000 |
DNA 3 kb | approx. 1000 |
RNA 1 kb | approx. 300 |
Antibodies (IgG) | approx. 150 |
Proteins in the lab | approx. 10–300 |
BSA (Albumin) | 69 |
Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
DNA | +++ | + | - | - | - | + | + |
RNA | + | +++ | - | - | - | + | + |
Proteins | - | + | +++ | +++ | +++ | + | + |
Detection System | |||||||
Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
Fluorescence | +++ | +++ | - | - | +++ | - | - |
+++: recommended | +: suitable | -: not |