Inhibitor cocktail for inhibition of a broad range of proteases. Inhibits serine proteases, esterases, metalloproteases, cysteine proteases, and trypsin-like proteases.
Recommended as cocktail for protease inhibition during tissue extraction.
Well water-soluble. The premixed powder may easily be reconstituted in 1 ml distilled water to form a approx. 100x inhibitor stock-solution.
Concentrations in stock-solution are:
AEBSF: 50 mM
Aprotinin: 15 μM
E-64: 100 μM
EDTA: 50 mM
Leupeptin: 100 μM
Gebruikstip | 1 vial is sufficient for inhibition of proteases from 1-2 g material. |
Appearance | white powder |
AEBSF hydrochloride | complies |
Aprotinin | complies |
E-64 | complies |
EDTA-Na2 | complies |
Leupeptin hemisulphate | complies |