Perfect separation behaviour in the gel. Protein ladders from Carl ROTH are acylated and pre-reduced, for optimal running behaviour in the gel. For each batch, intensity of bands is optimised and electrophoretically tested. All protein ladders are offered as ready-to-use solutions which may be applied directly to the gel.
Please note: Reliable size determination of proteins cannot be performed using prestained markers. For this, we recommend the use of an unstained marker.
Protein ladders including bicolour-prestained proteins of 10 up to 175 kDa. In order to visualise separation during the gel run and for better orientation in the gel, three proteins of approx. 10, 40 and 90 kDa have been prestained in blue in addition to the pink coloured bands. All proteins excel by having a very even running behaviour and form distinct bands.
ROTI®Mark BI-PINK is an ideal size ladder for controlling the gel run during SDS-PAGE and for reliable evaluation of transfer efficiency during Western Blot.
Fig.: 15 % SDS-PAGE (Tris)
Aantal proteïnebanden | 11 |
Aantal sporen | ~200/ml |
Kleuring | pink prestained |
Groottebereik | 10-175 kDa |
Bewijs in de westernblot | no detection |
Kwantificering | non recommendé |
Referentiebanden | 10 kD, 40 kD, 90 kD blue |
Verzending | ready-to-use |
Marker | ALL BLUE | BI-PINK | TRICOLOR | TRICOLOR XTRA |
Art. No. | 2242 (fig. 1) | 8269 (fig. 2) | 8271 (fig. 3) | 2244 (fig. 4) |
Size Range (kD) | 10-240 | 10-175 | 10-245 | 10-310 |
Number of Fragments | 12 | 11 | 12 | 12 |
Reinforced band at (kD) | 25, 72 | - | - | - |
Quantitation | not recomm. | not recomm. | not recomm. | not recomm. |
Staining | blue prestained | pink prestained | blue prestained | blue prestained |
Additional reference bands | - | 10 kD, 40 kD, 90 kD blue | 25 kD green, 75 kD red | 25 kD green, 75 and 310 kD red |
Detection in Western-Blot | no detection | no detection | no detection | no detection |
Number of Lanes in minigel | 200 / ml | 200 / ml | 200 / ml | 200 / ml |
Delivery | ready-to-use | ready-to-use | ready-to-use | ready-to-use |